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Effects on hypothalamic neurons In studies in a mouse model (C57BL/6, ob/ob mice) after peripheral exposure to semaglutide, the following were observed: Activation of POMC/CART neurons in the arcuate nucleus (ARC) of the hypothalamus c-Fos mapping showed a 3-5-fold increase in immunoreactivity compared to control Inhibition of NPY/AgRP neurons reduction in NPY and AgRP mRNA expression measured by qPCR Activation of neurons in the nucleus of the solitary tract (NTS) in the brainstem an area integrating signals from the vagus nerve The effect of these neuronal changes was a statistically significant reduction in food consumption by animals (p 99% binding)

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