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This mechanism raises the critical discontinuation question - does stopping GHK-Cu immediately halt enhanced collagen production returning synthesis rates to pre-treatment baseline, or does the improved dermal environment created during treatment continue supporting elevated collagen production through positive feedback mechanisms where better matrix structure itself promotes ongoing synthesis even without continuous peptide stimulation

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After deparaffinization, sections were stained with Mayer's hematoxylin solution (Wako, Tokyo, Japan) for 10 min, and then stained with eosin alcohol solution (Wako) for 4 min

Once insulin was discovered and insulin therapy initiated, insulin became the default therapyit was a wonder drug for kids with type one diabetes who were insulin deficientand physicians let their patients eat progressively more and more carbohydrates, trusting on the insulin to keep them healthy